LOCUS       Exported File           1865 bp ds-DNA    circular SYN 01-AUG-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pEZclone-NRS-Amp-Blunt-HC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 01, 2014 from SnapGene 1.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      205..234
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
                     /note="color: #ffffff"
     primer_bind     249..265
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    315..319
                     /note="Topo I Site"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    complement(320..331)
                     /note="Topo I Site"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(320..324)
                     /note="Topo I Site"
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     complement(374..390)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     terminator      403..434
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
                     /note="color: #ffffff"
     promoter        435..537
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             538..1398
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #00ff00
                     Cleavage site after base 606"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1422..1449
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
                     /note="color: #ffffff"
     rep_origin      complement(1461..183)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
       61 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      181 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag
      241 ttgattgtgt aaaacgacgg ccagtgtctg aggctcgctt cagtcctgat gcttgttatc
      301 gtattcgcgt gtcgccctta agggcgacac gcgaagtcga tgtcgcgtct gcctgaagtc
      361 aatactgacg atggtcatag ctgtttcctg tccatagcag aaagtcaaaa gcctccgacc
      421 ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
      481 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
      541 agtattcaac atttccgtgt cgcacttatt ccgttttttg cggcattttg ccttcctgtt
      601 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      661 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      721 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      781 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      841 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      901 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      961 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1021 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1081 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1141 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1201 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1261 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1321 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1381 ctgattaagc attggtaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc
     1441 ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1501 cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1861 gagtt
//